ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN: 2169-3048 Vol. 2 (7), pp. 484-489, July, 2013. © International Scholars Journals
Full Length Research Paper
Induced breeding of Clarias gariepinus using non-conventional method of abdominal incision
Yisa, T.A., S.L. Lamai, Tsadu, S.M. and R.J. Kolo
Department of Water Resources, Aquaculture and Fisheries Technology, School of Agriculture and Agricultural Technology, Federal University of Technology, Minna, Niger State, Nigeria.
*Corresponding author. E-mail: [email protected]. Tel: 08068169704.
Accepted 21 February, 2013
Abstract
The experiment was conducted using ripe and matured African catfish (Clarias gariepinus), size ranging from 400-650g average total body weight (TBW). They were procured from a private fish farm and transported in perforated 50 litre water holding capacity jerry can to Federal University of Technology, (F.U.T.) Minna, Bosso campus indoor hatchery fish farm. The fish samples were maintained for 2 weeks and observed for ripeness and maturity under optimum temperature and fed with 40% crude protein commercial diet with good water quality management before being used for breeding. Incision was made on the dorso-ventral part of the body through Abdominal Incision Method (AIM) to extract milt to fertilize eggs. Fecundity increased with body weight and hence larger fish had higher fecundity and significantly different (P<0.05) from each other. The spent spawners after operation survived on gradual recuperation post- surgery. The variation in incision length 3.40 cm was most effective and healing and recuperation was within 14 days post-surgery. The hatchlings bred from Conventional Method (CM) and AIM was maintained to determine survival and mortality rates for 12 weeks. Volume of milt extracted, percentage fertility and hatching differed significantly (P<0.05) between CM and AIM with highest volume of milt extracted from CM to be (0.86±0.006a). The recovery and re-use time for male C. gariepinus was 45 days post-surgery. Male C. gariepinus could be used up to 6 times in a year for the purpose of breeding. CM gave the highest percentage survival (75.20 %) though not significantly different (P>0.05), and with ±SEM (0.516) and SD (1.789) of the bred fingerlings that were managed for 12 weeks. The specie of C. gariepinus could be re-used for further genetic studies after abdominal incision breeding method and milt can be extracted without killing the male brood stock but proper and adequate feeding is necessary to hasten the maturation and development of the gonads. Variation in incision length 3.40 cm is recommended for abdominal incision to extract milt from male C. gariepinus for breeding.
Key words: Incision, milt, Clarias gariepinus, recuperation and breeding.