ISSN 2756-3685
International Journal of Enology and Viticulture Vol. 6 (2), pp. 001-004, February, 2019. © International Scholars Journals
Full Length Research Paper
Restriction analysis of rDNA regions to differentiate non-Saccharomyces wine species in mixed cultures
Capece A, Siesto G, Romaniello R and Romano P*
Dipartimento di Biologia, Difesa e Biotecnologie Agro- forestali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy.
Accepted 15 June, 2018
Abstract
The control of wine quality is related also to the knowledge of composition and dynamics of yeast -flora occurring throughout the vinification process. The restriction analysis of NTS and ITS regions was used to differentiate in mixed fermentation non-Saccharomyces species frequently encountered in the early fermentation phase, such as Hanseniaspora uvarum, Candida stellata and Metschnikowia pulcherrima. PCR-RFLP analysis of ITS region allowed to identify all the species inoculated in the grape must. The novelty of the protocol used is the application of PCR-RFLP analysis on yeast DNA isolated directly from grape must fermentation, without previous yeast colony isolation.
Key words: Non-Saccharomyces yeasts, NTS region, yeast mixed cultures.