ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (3), pp. 001-008, March, 2020. © International Scholars Journals
Full Length Research Paper
Assessment of the antifungal activities of polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya (Carica papaya L.) fruit
Rose Koffi-Nevry1*, Ama Lethicia Manizan1, Kablan Tano2, Yao Clément Yué Bi2, Mathias K. Oulé3 and Marina Koussémon1
1Biotechnology and Food Microbiology Laboratory, Department of Food Science and Technology, University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire.
2Food Biochemistry and Tropical Products Technology Laboratory, Department of Food Science and Technology, University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire.
3Université St-Boniface, Faculté des Sciences. 200 ave. de la Cathédrale, Winnipeg, MB, Canada.
Accepted 19 September, 2019
Abstract
The antifungal activity of the Polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya fruit was evaluated. The aim of this work was to show that the PHMGH can be used as a disinfectant for papaya preservation. Thirty one strains of fungi were isolated as Mucor sp., Botrytis sp., Penicillium sp., Geotrichum sp., Aspergillus sp. and Colletotrichum sp. Mucor sp. was the most isolated with a frequency of 52.77% followed by Botrytis (47.22%), Aspergillus and Penicillium (8.33%), Colletotrichum (5.56%) then, Geotrichum (2.78%). The antifungal activity of the PHMGH was tested through the determination of the minimal inhibitory concentrations (MICs) and the minimal fungicidal concentrations (MFCs). All the strains tested were sensitive to the disinfectant. However, the activity of PHMGH varies according to the strain tested. The values of the MIC and the MFC were between <0.01 and 1.9 mg/ mL. Aspergillus sp. was the least susceptible fungi with a MIC and MFC of 1.9 mg/ mL whereas the most sensitive to PHMGH were the genera Botrytis and Colletotrichum with a MIC <0.01 mg/ mL. No matter the strains of fungi studied, the MIC and MFC values were equal. The PHMGH could therefore be considered as a fungicidal agent that could serve for the preservation of papaya fruit after harvesting.
Key words: Antifungal activity, minimum inhibitory concentrations (MICs), minimum fungicidal concentrations (MFCs), papaya preservation, polyhexamethylene-guanidine hydrochloride.