ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (9), pp. 283-286, September, 2016. © International Scholars Journals
Full Length Research Paper
GARCINIA KOLA extract reduced lipopolysaccharide activation of macrophages using U937 cells as a model
Tebekeme Okoko1* and Ibiba F. Oruambo2
1Biochemistry Division, Department of Chemical Sciences, Niger Delta University, PMB 71, Yenagoa, Bayelsa State, Nigeria.
2Department of Chemistry, River State University of Science and Technology, Port Harcourt, Rivers State, Nigeria.
*Corresponding author. E-mail: [email protected]. Tel. +2348051069342.
Accepted 03 January, 2016
Abstract
The effect of GARCINIA KOLA heckel seed extract on the promonocytic cell line U937 activated by lipopolysaccharide (LPS) was investigated. 200 µl of U937 cells maintained in culture at 5 x 105 cells per ml was delivered into wells of a culture plate according to groups. Cells were pre-treated with 20 µl of 100 ng/ml phorbol myristate acetate (PMA) for 24, 48, or 72 h in order to transform them to the macrophage form. Following the PMA treatment, some cells were incubated with 100 µg/ml LPS (group C), while others (group D) were treated with 100 µg/ml LPS and 100 µl of 100 µg/ml of the G. KOLA methanolic extract. Other cells (groups A, and B) were incubated with neither LPS nor extract. 24 h later, the supernatants were analysed for the production of TNF-α and IL6 as indices for the activation of macrophages. The results show that the G. KOLA extract reduced the tendency of LPS to activate the cells. However, highest activation of LPS was observed when the cells were incubated with PMA for 48 h prior to the addition of LPS. This suggests that stimulation of U937 cells with PMA is necessary for the production of cytokines and that G. kola possesses considerable immunomodulatory activity.
Key words: Garcinia kola, lipopolysaccharide, phorbol myristate acetate, macrophage, cytokines.