Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (10), pp. 001-012, October, 2018. © International Scholars Journals

Full Length Research Paper

Biochemical and molecular characterization of Haemophilus influenza isolated from Riyadh, Kingdom of Saudi Arabia

Afaf I. Shehata*, Amal Abdulaziz Al-Hazani, Hesham Al-Aglaan and Hanan O. Al Shammari

Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.

Accepted 17 June, 2018

Abstract

The objective of this study focused on the prevalence of Haemophilus influenza to confirm the colonies of H. influenza on the basis of their growth requirements and serotype distribution. This study prepared 80 isolates of H. influenze isolated from five different sources (eye, ear, sputum (SP), lower genital tract (TA), and nasopharyngeal (NPA)) with different ages for infants and elderly persons. The phenotypic characteristics, which included the biotype, serotype, antibiogram and β-lactamase production, were applied by using APINH and minimum inhibitory concentration (MIC). Also, the study focused on the identification of selected serotype using PFGE analysis. The discussion of this study differentiates the age groups occurrence in the isolates, alongside with non-typeable strain versus the typeable ones and their percentages in the sample of isolates. This clustering of most strains in one PFGE pattern might be explained with the colonel population structure of the encapsulated H. influenza.

Key words: Haemophilus influenza, biotype, serotype, antibiogram, β-lactamase.