ISSN 2756-3685
International Journal of Enology and Viticulture Vol. 6 (9), pp. 001-008, September, 2019. © International Scholars Journals
Full Length Research Paper
Lipid-Lowering mechanisms of grape seed extract (VITIS VINIFERA L) and its antihyperlidemic activity
Sirichai Adisakwattana1*, Jeerasuk Moonrat2, Supatra Srichairat2, Chuliporn Chanasit3, Hathaichanok Tirapongporn3, Benjanut Chanathong1, Sathaporn Ngamukote1, Kittana Mäkynen1 and Suwimol Sapwarobol1
1Department of Transfusion Medicine, Faculty of Allied Health Sciences, The Medical Food Research and Development Center, Chulalongkorn University, Bangkok, Thailand, 10330, Thailand.
2Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330, Thailand.
3Undergraduate Program in Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand, 10330, Thailand.
Accepted 22 April, 2019
Abstract
Grape Seed Extract (GSE) has been targeted to promote beneficial health effects, especially the prevention of obesity and hyperlipidemia. In present study, we investigated the mechanisms of GSE on inhibition of lipid digestion and absorption. GSE significantly inhibited pancreatic lipase and cholesterol esterase in dose-dependent manner with the IC50 values of 3.71 ± 0.03 and 27.27 ± 4.12 mg/mL, respectively. In addition, GSE also inhibited the formation of cholesterol micelles, and bound to bile acid. The oral administration of GSE significantly decreased serum triglyceride and cholesterol in rat fed high fat emulsion through inhibition of lipid digestion and absorption. GSE may be a feasible therapeutic strategy for prevention and treatment of patients with hyperlipidemia and obesity.
Key words: Grape seed extract, bile acid binding, cholesterol micellization, pancreatic cholesterol esterase, pancreatic lipase, mechanisms.