Frontiers of Agriculture and Food Technology

Frontiers of Agriculture and Food Technology ISSN 7295-2849 Vol. 7 (5), pp. 001-009, May, 2017. © International Scholars Journals

Full Length Research Paper

The phytase from antarctic bacterial isolate, Pseudomonas sp. JPK1 as a potential tool for animal agriculture to reduce manure phosphorus excretion

Inkyung Park and Jaiesoon Cho*

Department of Animal Sciences and Environment, College of Animal Bioscience and Technology, Konkuk University, 1 Hwayang-dong, Gwangjin- gu, Seoul 143-701, Korea.

Accepted 14 January, 2017

Abstract

Intracellular phytase activity was found in the Antarctic bacterial isolate, JPK1. Based on 16S rRNA gene sequence analysis, the strain was related to Pseudomonas sp. The optimal activity of JPK1 phytase occurred at 50°C and pH 5.0 to 5.5. The enzyme was highly specific for phytate with little or no other phosphate conjugates. Enzyme activity was strongly inhibited by Cu ²⁺ and Zn²⁺, and completely inactivated by sodium dodecyl sulfate. The enzyme effectively liberated inorganic phosphate from wheat bran, a main feedstuff with high phytate content. The enzyme may be a good candidate for use as an environmental-friendly feed additive to enhance the nutritive value of phytate and reduce phosphorus pollution.

Key words: Antarctic, phytase, Pseudomonas sp., phytate, feed additive.