ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2756-3707 Vol. 7 (9), pp. 001-006, September, 2020. © International Scholars Journals
Full Length Research Paper
The use of biolistic inoculation of cassava mosaic begomoviruses in screening cassava cultivars from Ghana for resistance/susceptibility to cassava mosaic disease
Kwadwo Owusu Ayeh1* and Jon N. E. Ramsell2
1The Biotechnology and Nuclear Agriculture Research Institute (BNARI), Ghana Atomic Energy Commission (GAEC), P. O. BOX LG. 80 Legon-Accra Ghana.
2National Veterinary Institute. P.b._750 Sentrum, 0106 Oslo, Norway.
Accepted 11 June, 2020
Abstract
The resistance/susceptibility of 5 cassava cultivars from Ghana to infectious clones of two cassava mosaic viruses (ACMV-[CM] and EACMV/ACMV-[CM]) was investigated in this study. Plantlets of cassava cultivars were obtained using nodal cuttings initiated from tissue culture. These cassava cultivars were challenged with both DNA A and B components of the infectious clones named above using particle gun bombardment. The cassava cultivars showed varying degrees of susceptibility/resistance to the two infectious clones used. All symptoms of Cassava Mosaic Disease (CMD) observed were systemic in nature. Generally, all cassava cultivars used in this study exhibited varying degrees of recovery from virus infection. This report demonstrates the ability of using biolistic technology to screen cassava cultivars for tolerance/resistance and that it may be used to recommend resistant cultivars to the farming community.
Key words: Biolistic, cassava, ACMV, EACMV, inoculation.