ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-008, December, 2018. © International Scholars Journals
Full Length Research Paper
Optimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation
Sangeeta Negi1* and Rintu Banerjee2
1Biotechnology Section, Applied Mechanics Department, Motilal Nehru National Institute of Technology, 221 004 Teliarganj, Allahabad, India.
2Agriculture and Food Engineering Department, Microbial Biotechnology, 721302 Kharagpur, India.
Accepted 27 September, 2018
Abstract
Concomitant production of amylase and protease by Aspergillus awamori nakazawa MTCC 6652 was enhanced in a single fermentation by media engineering and optimization of other important parameters. Wheat bran was considered as a suitable substrate for production of amylase and protease by A. awamori in a single fermentation as none of the additional substrate such as powder of peanut, corn, soybean seeds and sunflower seeds was effective in enhancing the concurrent production of enzymes further. Optimum amylase yield of 4528.4 ± 121U/gds and protease yield of 250.4 ± 10 U/gds was achieved with wheat bran to Czapek-dox ratio of 1:1.5 (w/v), 96 h incubation, 35˚C temperature, pH 5.5 and 85% relative humidity. Media engineered with 1% casein and 1% starch solution increased yield of amylase and protease by 2.07 fold (9386.5 ± 101 U/gds) and 3.73 fold (934.8 ± 67 U/gds), respectively, therefore, considered as the most suitable media for concomitant production of amylase and protease by A. awamori in a single fermentation.
Key words: Amylase, protease, solid state fermentation, optimization.