ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Production of recombinant cellulase enzyme from Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)
Ayman S. Daba1, Ghada A. Youssef2*, Sanaa S. Kabeil1 and Elsayed E. Hafez1
1Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt.
2Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.
Accepted 19 January, 2019
Abstract
Successful utilization of Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366) mushroom as a type of edible locally isolated mushroom in Egypt at the Mushroom Research Center (Mubarak City for Scientific Research and Technology Applications), to produce extensive hydrolyzing cellulase complex enzymes. This hydrolysis was approached in submerged culture supplemented with avicel PH101 as a substrate for endo-,exoglucanase production. The avicel concentration 6% yielded the maximum enzyme activities (2.46, 1.80 U/ml) for both endo-and exoglucanase activities on basal medium at 27°C, initial pH value of 5.5 for 12 days on rotary shaker (180 rpm) incubation period. Cellulase enzyme was amplified using specific PCR and the amplicone was cloned using TOPO TA cloning vector. The cellulolytic activity of the recombinant protein was examined and high activity was obtained compared with the standard ones. The avicel was used as a sole carbon source in the fermentation medium and the results revealed that, avicel induced the cellulolytic activity of the examined organism compared with those grown on medium deficient of avicel.
Key words: Pleurotus ostreatus, mushroom, avicel, recombinant cellulolytic enzymes.