ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Investigation of accessory gene regulator (agr) in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran
Hassan Momtaz1*, Elahe Tajbakhsh2, Behnam Abbasian3 and Manochehr Momeni4
1Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
2Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord branch, Shahrekord,
Iran. 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch,
Shahrekord, Iran.
4Department of Biotechnology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
Accepted 24 February, 2014
Abstract
Mastitis is one of the common diseases of dairy cattle and an inflammatory response of the mammary glands tissue. Mastitis causes considerable loss to the dairy industry. Among several bacterial pathogens that can cause mastitis, S. aureus is probably the most lethal agent because it causes chronic and deep infection in the mammary glands that is extremely difficult to be cured. The present study was to detect agr group genes in the S. aureus isolated from 360 mastitis milk samples in Chaharmahel va Bakhtiari and Isfahan provinces of Iran via PCR by using specific primers. Among 360 milk samples, 86 samples contained 1250 bp fragment of the 23srRNA gene, 10 samples contained agrI gene, 42 samples contained agrII gene, 19 samples contained agrIII gene and 15 samples contained agrIV gene.
Key words: Bovine mastitis, S. aureus, agr group genes, PCR, Iran.