Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research Vol. 2017

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2017 International Scholars Journals

Full length Research Paper

Sequence analysis of the groEL gene and its potential application in identification of pathogenic bacteria

Hu Yushan^1,2, Luo Lei^1,2, Liu Weijia^1,2 and Chen Xiaoguang¹*

¹Department of Pathogenic Biology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.

²The Center for Disease Control and Prevention of Guangzhou, Guangzhou 510080, China.

Accepted 22 July, 2017

Abstract

This study aims to develop a rapid method for identifying pathogenic bacteria based on the sequence difference of pathogenic bacteria groEL gene. A pair of universal degenerate primers was designed to amplify the groEL genes of 34 major species of 16 genus’s of pathogenic bacteria. The conservation, variation and the interspecies phylogenetic relations of the groEL gene sequences were informatically analysed. Our results showed that the groEL gene fragments of the selected pathogenic bacteria could be amplified using the degenerate primer. The results of the sequence comparative study indicated that the groEL gene is characterized by conservation and variation, while the variation regions are distributed separately among the conserved regions, just like the mosaic. This conservation and variation coexistence of pathogenic bacteria groEL genes could provide a solid basis for the rapid identification of pathogenic bacteria and the development of a diagnostic microarray.