ISSN 2375-074X
African Journal of Plant Breeding ISSN 2375-074X Vol. 4 (4), pp. 001-006, November, 2017. © International Scholars Journals
Full Length Research Paper
Genetic variation of 1RS arm between sibling wheat lines containing 1BL.1RS translocation
Tan FeiQuan1, Fu ShuLan1, Tang ZongXiang1, Ren ZhengLong1,2* and Zhang HuaiQiong1
1State Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Ya’an, Sichuan 625014, China.
2School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Accepted 06 June, 2017
Abstract
3 sets of sibling 1BL.1RS translocation wheat lines (each set containing 2 sibling lines) derived from 3 different F4 single plant. Between 2 sibling wheat lines, different resistance to powdery mildew was observed. In addition, different bands in -gliadin mobility zone between 2 sibling wheat lines were also significantly observed by acid polyacrylamide gel electrophoresis (A-PAGE). However, the nucleotide sequence of / -gliadin precursor gene cloned from 2 sibling wheat lines were 100% similarity. Polymerase chain reaction (PCR) analysis using 180 wheat microsatellite markers dispersed on 7 homeologous groups of wheat indicated that little genetic variation occurred in wheat A, B and D genomes of these sibling wheat lines. Structural variation of 1BL.1RS chromosome arm was detected by PCR analysis. Different rye-specific repetitive DNA exhibited different variation. The different structure of 1RS chromosome arms may be related to the genetic variation between the 2 sibling wheat lines. The results lead us to think that the variation of 1RS arm within a species should be utilized in wheat breeding program. To detect variation of repetitive DNA can provide better understanding of the genetic variation of 1BL.1RS translocation and will be useful for utilization of 1RS arm in wheat breeding program.
Key words: 1BL.1RS translocation, repetitive DNA, sibling wheat lines, gentic variation.