ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (1), pp. 235-240, January, 2016. © International Scholars Journals
Full Length Research Paper
A study of the optimum culture conditions and factors involved in maximum production of glucoamylase
Lagzouli Mohamed1*, Mennane Zakaria 1, Aitounejjar Ali2, Wafae Senhaji3, Ouhssine Mohamed1, Elyachioui Mohamed1, Berny EL Hassan1 and Jadal Mohamed
1Laboratory of Microbial Biotechnology, University IBN TOFAIL, Department of Sciences, BP 133, 14000 Kenitra-Morocco.
2National Institute of Agronomic Researches, INRA, BP: 589 Settat 26000, Morocco.
3Laboratoire de Recherche et d'Analyses Médicales de la Gendarmerie Royale, avenue Ibn Sina-Agdal-Rabat, B.P 8087, 10000, Morocco.
*Corresponding author. E-mail: [email protected].
Accepted 16 March, 2007
Abstract
Candida famata was isolated from traditional Moroccan sourdough. It exhibited high glucoamylase and biomass production. Starch induces high glucoamylase production C. famata with maximum glucoamylase activity at 5 g/L. Glucose stimulates good production in biomass but strongly inhibits glucoamylase production. Among the sources of nitrogen tested, yeast extract and the (NH4)2HPO4 gave maximum glucoamylase and biomass after 72 h of incubation in liquid medium at 30°C, pH 5 and 105 rpm.
Key words: Glucoamylase, yeast, optimization, biotechnology, Candida famata.