ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Separation and identification of lipase - producing strains and immobilized transesterification efficiency
Jing Li1, 2, Shu Zhang1, Guanghua Liu1, Rui Zhao1, Hui Xu, Dairong Qiao1 and Yi Cao1*
1Microbial and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, 610064, China.
2College of Material and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu, 610059, China.
Accepted 26 January, 2014
Abstract
12 strains of lipase - producing were isolated from 36 samples collected from oil - contaminated soil of machinery - repair plant, dining room, and vegetable market, respectively, of which strain (3 - 2) had the highest hydrolytic activity (17.1 U/ml). Immobilized cell of strain 3 - 2 onto sodium alginate and immobilized crude enzyme solution of strain (3 - 2) onto Sodium alginate were respectively used in two transesterification programs to transfer the Fatty acid methyl ester (FAME). Results showed that both immobilized crude enzyme solution and cell were of high transesterification efficiency for Transesterification Program I, respectively 32.72 and 26.84%. Cycle test done in this Program found that immobilized crude enzyme solution and cell still keep some transesterification efficiency after being recycled thrice at least. Meanwhile, hydrolytic activity of crude enzyme solution had some correlation with transesterification efficiency. However, hydrolytic activity of immobilized cell did not have a clear correlation with the latter. Strain (3 - 2) belonged to Sphingobacterium multivorum. Up to now, the transesterification research has not been reported.
Key words: Lipase, crude enzyme solution, bacterial cell, immobilized, transesterification.