ISSN 2375-1134
African Journal of Agriculture ISSN 2375-1134 Vol. 7 (6), pp. 001-005, June, 2020. © International Scholars Journals
Full Length Research Paper
Comparative analysis of genomic DNA isolation procedures; hexa decyltrimethylammonium bromide (CTAB) and liquid detergent (morning fresh®) methods from Samsorg 41 (icsv 400)
Inuwa H. M.1, Aimola I. A.2* and Inuwa L.1
1Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria.
2Department of Biochemistry, National Research Institute for chemical Technology, Zaria, Nigeria.
Accepted 22 February, 2020
Abstract
Genomic DNA was prepared from germinating seeds of a variety of sorghum in Nigeria (Samsorg 41) using two different isolation protocols (CTAB and morning fresh® method). Highest quantity of DNA was obtained from Samsorg 41 using the morning fresh® method. Genomic DNA obtained using this method had a much lower purity as compared with that obtained using the CTAB method. Both methods produced genomic DNA of high integrity with minimum shearing.
Key words: Genomic DNA, CTAB, morning fresh®, isolation, Samsorg 41, Nigeria.