ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Effects of Rhizobium innoculation, lime and molybdenum on nitrogen fixation of nodulated Phaseolus vulgaris L.
Sylvie Bambara and Patrick A. Ndakidemi*
Faculty of Applied Science, Cape Peninsula University of Technology, Cape Town Campus, Keizersgracht, P.O. Box 652, Cape Town 8000, South Africa.
Accepted 9 March, 2016
Abstract
A field and glass house experiment was conducted with the aim of evaluating Rhizobium inoculation, molybdenum (Mo) and lime supply on growth and nitrogen fixation of nodulated Phaseolus vulgaris. The experiment was laid in a split-split plot design. The experimental treatments consisted of 2 levels of Rhizobium inoculation (with rhizobia and without rhizobia) 3 levels of Mo (0, 6 and 12 g kg-1 of seeds) and 3 levels of lime (0, 2 and 3 t ha-1). Rhizobium inoculation showed significant increase in dry matter yield of different organs and decreased 15N values in all organs assessed, thus resulting to improved % nitrogen derived from atmosphere (%Ndfa) in all organs and the amount of N derived from fixation. Molybdenum and lime were significantly effective in promoting plant growth in most organs and decreased 15N values in all organs for glasshouse and field experiment. Lowest 15N values were recorded in Mo and lime supplied at the highest rates of 6 and 12 g kg-1 of seeds and 2 and 3 t lime per ha, respectively. Molybdenum and lime application also significantly improved %Ndfa in all organs and N derived from N-fixation in most organs. Significant responses in N nutrition were also reported in treatments involving the combination of Rhizobium x Mo x lime with better result being recorded in treatments involving Rhizobium inoculation and higher levels of Mo and lime.
Key words: Nodulated root, 15N, %Ndfa, total N.