International Journal of Agricultural Sciences

International Journal of Agricultural Sciences ISSN 2167-0447 Vol. 6 (3), pp. 959-962, March, 2016. © International Scholars Journals

Full Length Research Paper

A study of Lactobacillus sp. with Moringa oleifera leaves extract for food supplement

*¹Bowo Sutiyoso, ¹Ali Wiyogo and ²Fauzi Yusuf

^*1Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia.

²Central Laboratory of Life Sciences, Brawijaya University Malang, Indonesia.

*Corresponding author email: [email protected]

Accepted 22 December, 2015

Abstract

A study was carried out to find out the best combination treatment between Moringa oleifera leaf concentration and filler to produce encapsulated Lactobacillus food supplement. Inoculation of Lactobacillus on to media containing different types of amino acid and mannitol to promote the growth of organism showed a promising result. The use of proline (0.45% w/v), lysine (0.30% w/v) and mannitol (0.75% w/v) reduced the lag phase from 12 hours to 8 hours and reached the stationer phase after 13 hours of incubation. Encapsulation of Lactobacillus with liquid medium containing 8% Moringa oleifera leaf and 15% maltodextrin then dried with spray dryer producing a promising product containing protein 27.37%; vitamin A 159.60 mg/g vitamin C 50.95 mg/100g; calcium 773.45 mg/100g. On allowing standing the encapsulated organism in nutrient broth at 30⁰C showed that the viable cell count only reduced by 5% and the growth rate was similar with that of fresh culture.

Keywords: Lactobacillus sp., Moringa oleifera, probiotics, coconut milk.