ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 4 (6), pp. 333-336, June, 2017. © International Scholars Journals
Full Length Research Paper
A study of possible formation of micronuclei in red blood cells of Hipostomus plecotomus
Carlos Augusto Borba Meyer Normann1, José Cláudio Fonseca Moreira2 and Valesca Veiga Cardoso1*
1Centro Universitário Metodista - Porto Alegre - RS –Brazil.
2Department of Biochemistry-ICBS; UFRGS - Porto Alegre - RS - Brazil.
*Corresponding author. E-mail: [email protected].
Accepted 06 April, 2016
Abstract
The present work aims to evaluate the impact of potassium dichromate in armored catfishes’ (Hypostomus plecotomus) erythropoiesis, using piscine micronucleus test. Armored catfishes (n = 30) were subjected to 12 mg/L of potassium dichromate, with an equal control group (n = 30). For each 2,000 red blood cells of animals subjected to treatment, it was possible to observe 8.25 ± 0.02% cells with micronuclei and 17 ± 0.005 mg/kg of total chrome in tissues, detected through atomic absorption spectrophotometry. In the control group, the average was 0.75 ± 0.03% for micronuclei and 1.08 ± 0.04 mg/kg of chrome in tissues. The results confirm the clastogenic effect of potassium dichromate. The sensibility to this ion and the economic relevance of H. plecotomus show that armored catfish can be used to monitor acute effects of metallic pollutant spills in freshwater.
Key words: Piscine micronucleus test, aquatic pollution, potassium dichromate, Hypostomus plecotomus.