Advances in Aquaculture and Fisheries Management

Advances in Aquaculture and Fisheries Management ISSN: 9424-2933 Vol. 7 (4), pp. 001-007, April, 2019. © International Scholars Journals

Full Length Research Paper

Distinguishing proof of the S-genotypes of a few sweet cherry (Prunus avium L.) cultivars by AS-PCR and fertilization

*Wang Lee and Chain V. Zackie

Department of Fisheries Sciences, Faculty of Agriculture, Sichuan University, Chengdu, China.

Accepted 20 January, 2019

Abstract

Sweet cherry cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. PCR-based S-allele typing system for sweet cherry cultivars has been recently developed. It has been reported that all known self-incompatibility (S) alleles of sweet cherry were more than 16 based on the AS-PCR analysis. In this work, two sets of AS-PCR primers that were designed based on conserved domains of cDNA sequences of S-RNases has been used to characterize the S-genotype of 38 sweet cherry cultivars, including 15 cultivars whose S-genotype had not been previously described. Pollination test was also performed to validate the PCR results, and the consistent results were got. A wide variation in the frequency of S-alleles in the Sweet cherry germplasm was observed. S₃ was the most common in the cultivars evaluated, and S₇, S₁₀-S₁₆, S₂₃-S₂₅ as rare allele was not found in China geographical areas in our study.

Key words: Self-incompatibility, Prunus avium, S-RNase, AS-PCR, pollination test.