International Journal of Animal Breeding and Genetics

International Journal of Animal Breeding and Genetics Vol. 7 (1), pp. 001-005, January, 2018. © International Scholars Journals

Full Length Research Paper

Study on ultrastructure of Leishmania major and Lizard leishmania

Bahram Kazemi^1,2*, Mohammad Hassan Heidari^1,3, Mehdi Naderi², Abbas Piryaei ^{1, 3}and Mohammad -Reza Nazari -Pouya²

¹Cellular and Molecular Biology Research Center -Shahid Beheshti University M.C., Tehran, I.R. Iran.

²Parasitology Department - Shahid Beheshti University, M.C., Tehran, I.R. Iran.

³Anatomy Department - Shahid Beheshti University M.C., Tehran, I.R. Iran.

Accepted 16 October, 2017

Abstract

We compared ultrastructure of Lizard Leishmania and Leishmania major promastigotes by electron microscope. Leishmania major (MRHO/R64/Nadim1) and Lizard leishmania promastigotes were mass cultured, collected by centrifugation and washed by PBS buffer three times, the ultrastructural features of both Leishmania promastigotes were observed by transmission electron microscope Their subpellicular microtubules diameter and cytoplasmic membrane thickness are identical, but there is difference in two parasites in respect of distance between kinetoplast and flagella. We could not find golgi apparatus in Lizard Leishmania.

Key words: Lizard Leishmania, Leishmania major, Transmission Electron Microscope