Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research Vol. 2005

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2005 International Scholars Journals

Full Length Research Paper

Temperature effect on dextransucrase production by Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant

Mariana CORTEZI¹, Rubens MONTI² and Jonas CONTIERO^1*

¹Departamento de Bioquímica e Microbiologia, Instituto de Biociências de Rio Claro/ UNESP, São Paulo, SP, Brasil.

²Departamento de Alimentos e Nutrição, Faculdade de Ciências Farmacêuticas UNESP, Araraquara, São Paulo, SP, Brasil.

Accepted 13 August, 2004

Abstract

Temperature (23 to 31°C) and sucrose concentration (3 and 4%) effects on dextransucrase production by Leuconostoc mesenteroides NRRL B 512 (F) and Leuconostoc mesenteroides FT 045 B were studied. The conditions in all fermentations were: total reaction volume 2 L, 132 rev. min-1, 0.5 vvm and pH 6.0. The optimum temperature for enzyme yield for strain NRRL B 512 (F) was 23°C, where at 8-h fermentation was possible to achieve 49.3 DSU/mL. When FT 045 B strain was utilized, 3.2 DSU/mL was obtained at temperature 23 to 25 °C.

Key words: Dextran, dextransucrase, Leuconostoc mesenteroides NRRL B512 (F), Leuconostoc mesenteroides FT045 B, temperature, enzyme activity.