ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Study on transformation of cowpea trypsin inhibitor gene into cauliflower (Brassica oleracea L. var. botrytis)
Lv lingling1*, Lei Jianjun2, Song-Ming3, Li Liyun4, Cao Bihao5
1Southern Subtropical Crop Research Institute, Zhan Jiang, Guang Dong , 524091 China.
2South China Agricultural University, Guang Zhou, Guang Dong, 510642 China.
3Southwest Agricultural University, Chong Qing, 400716 China.
4Agriculture & Industry & Commerce Secondary professional School, Zhan Jiang, Guang Dong , 524091China.
Accepted 26 August, 2004
Abstract
Cowpea Trypsin Inhibitor (CpTI) gene was transferred into cauliflower by agrobacterium-mediated transformation method, and 14 transgenic cauliflower plants were obtained. Cotyledons and hypocotyls were used as explants. The putative transformants were assayed by PCR and Southern blotting analysis. The results indicated that CpTI gene was transferred into cauliflower successfully. The result of preliminary insect-resistant assay showed that the transgenic plants were more resistant to Pieris rapae than non-transgenic plants.
Key words: cauliflower (Brassica oleracea L. var. botrytis), agrobacterium, mediated genetic transformation CpTI gene, transgenic plant.