ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (5), pp. 001-003, May, 2020. © International Scholars Journals
Short Communication
Optimization of thermostable alkaline protease production from species of Bacillus using rice bran
Krishna Suresh Babu Naidu, Kodidhela Lakshmi Devi*
Department of Biochemistry,Sri Krishna DevarayaUniversity,Anantapur, AndhraPradesh, India.
Accepted 19 May, 2020
Abstract
A protease producing microorganism was isolated from soil collected from a detergent industry and identified as Bacillus species. Isolate K -30 produced thermostable alkaline protease utilizing rice bran. The optimum conditions for protease activity was 55°C at pH 9 with 4% inoculum in the medium containing 1% rice bran after 96 h of incubation. Beef extract, tryptone and yeast extract were good nitrogen sources while lactose, starch, and sucrose were suitable for enzyme production. The extracellular production of the enzyme, its thermostable nature and compatibility with most commercial detergents are features which suggest its application in the detergent industry.
Key words: Alkaline protease, rice bran, thermostability, detergent.