ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
Generation of a cDNA library reveals the presence of a Ty-1/copia transposon in Phellinus rimosus (Hymenochaetaceae, Basidiomycota)
Luis Cesar Maffei Sartini Paulillo1, Sizenando José de Andrade Junior2, Abelmon da Silva Gesteira3, Marco Brotto4,5 and Aristóteles Góes-Neto2
1Faculdade de Tecnologia e Ciências - FTC, Mestrado Profissional em Bioenergia. Av. Luis Viana Filho, 8812, Pituaçu,
Salvador, CEP 41.741-490, BA - Brasil.
2PPG Biotecnologia UEFS/Fiocruz-BA. Av. Transnordestina s.nº, Novo Horizonte, Feira de Santana, – CEP 44.036-900, BA – Brasil.
3Embrapa Mandioca e Fruticultura Tropical. Rua Embrapa, s/nº. Cruz das Almas, CEP 44380-000, BA – Brasil.
4Muscle Biology Research Group-MUBIG, Schools of Nursing, Medicine, and Biological Sciences, University of Missouri-Kansas City, Kansas City, 64108. Missouri. USA.
5International Visiting Professor and Researcher, Conselho Nacional de Pesquisa-CNPq and Faculdade de Tecnologia e Ciências-FTC, Salvador, Brasil.
Accepted 22 February, 2011
Abstract
Lignocellulolytic fungi have significant potential as bio-convertors with a particular capacity to degrade recalcitrant natural composts, such as lignin. We generated a cDNA library of a lignocellulolytic fungus, the Phellinus rimosus (Hymenochaetaceae, Basidiomycota), and a typical species of polypore mushroom from the Brazilian semi-arid region. To the best of our knowledge, this is the first report ever to describe the existence of a Ty1-copia retrotransposon in the P. rimosus species. Given the taxonomic complexity in the Brazilian semi-arid region, our findings will be useful as they provide a molecular identity to this important parasite species of the semi-arid region of Brazil.
Key words: Lignocellulolytic fungi, biotechnology, Phellinus rimosus, Ty-1 copia, Brazilian semi-arid region, molecular marker.