African Journal of Plant Breeding

African Journal of Plant Breeding ISSN 2375-074X Vol. 5 (3), pp. 001-005, March, 2018. © International Scholars Journals

Full Length Research Paper

Structural features of a cytoplasmic male sterility source from Helianthus resinosus, CMS RES1

F. Ardila^1,2, M. M. Echeverría ^1,3*, R. Rios² and R. H. Rodríguez³

¹Ex Aequo asbl, Rue Locquenghien, 41 B-1000 Brussels, Belgium.

²Instituto de Genética CICV y A, CNIA, INTA. C.C. 25 (1712) Castelar, Buenos Aires, Argentina.

³Unidad Integrada: Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata - Estación Experimental INTA Balcarce. C.C. 276 (7620) Balcarce, Buenos Aires, Argentina.

Accepted 22 November, 2017

Abstract

Comparisons in the structural organization of mitochondrial DNA between a cytoplasmic male sterility source from Helianthus resinosus (CMS RES1) and HA89 fertile line have been performed surrounding coxIII and atp9 genes. Differences have also been recognized by PCR analyses between HA89 (CMS RES1) and either HA89 (CMS PET1) or HA89 (CMS MAX1), indicating that the molecular basis of their sterility mechanism is different. To be specific, RES1 lacks orfH522, which is present in the mitochondrial DNA of PET1 and MAX1.

Key words: Helianthus annuus, Helianthus resinosus, sunflower, cytoplasmic male sterility, CMS, coxIII, atp9.