ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 4 (1), pp. 293-298, January, 2017. © International Scholars Journals
Full Length Research Paper
Evaluation of the cytotoxicity interactions between epirubicin and daunorubicin in HeLa cell cultures
Gül Özcan Arıcan1*, Uğur Serbes1 and Ercan Arıcan2
1Istanbul University, Science Faculty, Biology Department, Turkey.
2Đstanbul University, Science Faculty, Molecular Biology and Genetics Department, Turkey.
*Corresponding author. E-mail: [email protected].Tel: 00212 455 57 00 ext. 15093. Fax: 00 212 528 05 27.
Accepted 11 March, 2016
Abstract
Epirubicin and Daunorubicin, antibiotics which are derivative of anthracyclines, are used on cancer chemotherapy. In this study, Epirubicin and Daunorubicin effects on cell kinetics parameters were examined both single and in combination on HeLa cell culture that was taken from human servical carcinoma. The experiments tested by using IC90 doses of Epirubicin and Daunorubicin (0.5 µg/ml and 0.1 µg/ml, respectively) were applied for 24 and 48 h. Cell kinetics parameters such as growth rate (WST-1 colorimetric assay), mitotic and apoptotic index were applied to identify cytotoxicity that was formed by drugs. In addition, DNA degradation was examined in agarose gel electrophoresis. The cytotoxic effect of drugs appeared significant decline of growth rate and mitotic index, and significant increase of apoptotic index of HeLa cell line (p<0.05 - 0.001). The enhancement of the cytotoxicity to Daunorubicin was closely associated with the DNA degradation.
Key words: HeLa cells, cell kinetics, DNA, apoptosis, epirubicin, daunorubicin.