ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (1), pp. 001-007, January, 2019. © International Scholars Journals
Full Length Research Paper
Recombinant matrix extracellular phosphoglycoprotein (MEPE) can promote mineralization in vitro
Ai Lin1, Wang Huiyuan2*, Hu Hua3, Liu Yanqing4, Ma Tiehua1 and Zhai Jingyun1
1Department of Stomatology, People’s Liberation Army No. 12 hosipital, Kuonabazha road 13, Kashi, 844200, China.
2Department of Stomatology, People’s Liberation Army No. 323 hosipital, Jianshe West Road 66, Xi’an, 710048, China.
3Department of Institution, People’s Liberation Army No. 12 hosipital, Kuonabazha road 13, Kashi, 844200, China.
4Out-patient Department, People’s Liberation Army No. 12 hosipital, Kuonabazha road 13, Kashi, 844200, China.
Accepted 17 January, 2019
Abstract
The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone and teeth mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE after cloning its cDNA from the cDNA library of a human brain cDNA library. Using this anti-body, we analyzed the distribution of MEPE in dog dental germ tissue by immunohistochemistry. In these specimens, MEPE was predominantly expressed by odontoblast cells and predentin, but not by dental pulp cells. Furthermore we used von kossa staining. And the results suggested that MEPE could induce mineralization and we propose that this protein has a potential effect on dental rehabilitation.
Key words: Matrix extracellular phosphoglycoprotein, mineralization Von kossa.