ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Early detection of ovarian carcinoma by proteome profiling based on magnetic bead separation and matrix-assisted laser desorption/ionization time of flight mass spectrometry
Dong-Mei Fan, Hui-Rong Shi *, Zhi-Min Chen, Qing-Hua Wu, Hui-Na Liu and Rui-Tao Zhang
Department of Obstetrics and Gynecology, The First Affiliated Hospital, Zhengzhou University, 1 Jianshe Road, Zhengzhou City, Henan, 450052, China.
Accepted 13 April, 2015
Abstract
In this study, affinity purification with magnetic beads and matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was used to screen candidate biomarkers of ovarian carcinoma. Mass spectrometry compiled from serum of 30 cases of ovarian carcinomas was compared with that from serum of 10 cases of benign group and13 cases of normal group. Interesting peaks were further sieved from ascites and cystic fluid. Peptides were deduced by searching in http://us.expasy.org/tools/tagident.html and were detected by immunohistochemistry and enzyme-linked immunosorbent assay. Results showed that two peaks of 2881 and 2897Da elevated in the serum, malignant ascite and cyst fluid of cancer group when compared with normal and benign groups were deduced as gastrin -releasing peptide which was higher in the malignant tissues and serum than other two groups. It was concluded that gastrin-releasing peptide in serum might be a potential marker for ovarian carcinoma.
Key words: Gastrin-releasing peptide, matrix-assisted laser desorption/ionization time of flight mass spectrometry, magnetic bead, and ovarian carcinoma.