ISSN 2756-3685
International Journal of Enology and Viticulture Vol. 1 (1), pp. 001-003, January, 2014. © International Scholars Journals
Full Length Research Paper
Method for RNA extraction from grapevine berry skins
Richard Lim, Yang Lee and Feng Yamaha
Center for Viticulture and Enology, College of Food Science & Nutritional Engineering, Tsinghua University, P.O. Box 400165, Beijing, China.
Email: [email protected]
Accepted 12 December, 2013
Abstract
RNA extraction is the foundation of modern molecular biology. It is difficult to extract high quality RNA from grapevine tissues, especially in cases where polysaccharides and polyphenols are rich. In this paper, the modified cetyltrie thylammnonium bromide (CTAB), sodium dodecyl sulphate (SDS) and guanidine isothiocyanate methods were applied to extract high purity and integrity RNA from grapevine green and mature berry skins. Highly-grade RNA was isolated by using modified CTAB method and it is suitable for the demands of further molecular biological research such as reverse-transcription polymerase chain reaction (PCR) (RT-PCR), cDNA library construction and Northern hybridization.
Key words: Grapevine berry skins, RNA extraction, reverse-transcription polymerase chain reaction (RT-PCR).