Advanced Journal of Microbiology Research

ISSN 2736-1756

Advanced Journal of Microbiology Research Vol. 2017

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2017 International Scholars Journals

Full Length Research Paper

Antisickling property of Carica papaya leaf extract

N.  O. A. Imaga1*, G. O. Gbenle1, V. I. Okochi1, S. O. Akanbi1, S. O. Edeoghon1, O.  V. Oigbochie1, M. O. Kehinde2 and S. B. Bamiro3

1Department of Biochemistry, College of Medicine, University of Lagos, P. M. B. 12003, Idi-Araba, Lagos State, Nigeria.

2Department of Medicine, College of Medicine, University of Lagos, P. M. B. 12003, Idi-Araba, Lagos State, Nigeria.

3Department of Obstetrics and Gynecology, College of Medicine, University of Lagos, P. M. B. 12003, Idi-Araba, Lagos State, Nigeria.

Accepted 10 February, 2017

Abstract

Sickle cell disease (SCD) results from a mutation in the hemoglobin inside the red blood cells, where a glutamic acid at position 6 is replaced by a valine. Many phytomedicines have been identified as potential antisickling agents, stemming from reported usage as ethnomedicines by the local folk. This research examined methanolic leaf extracts of Carica papaya L. (Caricaceae) for possible in vitro antisickling and membrane-stabilizing activities involving the use of positive (p-hydroxybenzoic acid 5 mg/ml) and negative (normal saline) controls for the antisickling experiments and osmotic fragility test on Hbss red blood cells obtained from non- crisis state sickle cell patients. Fragiliograms indicated that the plant extract reduced hemolysis and protected erythrocyte membrane integrity under osmotic stress conditions. Pretreatment of SS cell suspensions with C. papaya leaf extract inhibited formation of sickle cells under severe hypoxia, with only 0 - 5% sickle cells at 40 min compared with untreated SS cell suspensions which had over 60% sickle cells. These results indicate the feasibility of C. papaya as an attractive potential candidate for SCD therapy.

Key words: Antisickling, membrane-stabilizing, sickle cell disease, erythrocyte fragility, Carica papaya, toxicity profile.

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S. O. Akanbi on Google Scholar S. O. Akanbi on Pubmed S. O. Edeoghon on Google Scholar S. O. Edeoghon on Pubmed N. O. A. Imaga* on Google Scholar N. O. A. Imaga* on Pubmed M. O. Kehinde and S. B. Bamiro on Google Scholar M. O. Kehinde and S. B. Bamiro on Pubmed V. Oigbochie on Google Scholar V. Oigbochie on Pubmed V. I. Okochi on Google Scholar V. I. Okochi on Pubmed G. O. Gbenle on Google Scholar G. O. Gbenle on Pubmed