Advanced Journal of Microbiology Research

ISSN 2736-1756

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-007, March, 2019. © International Scholars Journals

Full Length Research Paper

The in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus from hospital currency notes

Adegoke Anthony A. and Okoh Anthony I.

Department of Biochemistry and Microbiology, Applied and Environmental Microbiology Research Group (AEMREG), University of Fort Hare, Alice, South Africa.

Accepted 03 February, 2019

Abstract

We determined the in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus isolates and presumptive roles of plasmid DNA using standard microbiological procedures. A total of 32 paper naira notes from seven different hospital sections (mostly from the patients) were analyzed. Thirteen notes (40.6%) gave isolates of S. aureus and subsequent resistant range of between 62.5 and 100% to frequently prescribed antibiotics. The same set of isolates showed three (23.1%) to be susceptible and ten (76.9%) to be resistant to vancomycin. Five of the latter ten isolates produced beta lactamase and became susceptible to some of the multidisc antibiotics and vancomycin, after the plasmid curing. This presumptively reflects important role of plasmid DNA in conferring resistance to microorganisms as a means of their adaptation especially in the vancomycin resistant S. aureus (VRSA). The increasing resistance to vancomycin could cause major problems to public health as it was often regarded as the last line of defense against nosocomial and community based S. aureus infection.

Key words: Vancomycin, multidrug, plasmid, beta lactamase, antibiotic resistance.