ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-004, February, 2019. © International Scholars Journals
Full Length Research Paper
Alteration of intercellular adhesion molecule-1 (ICAM-1) expression in the liver and serum level of HBV DNA in chronic hepatitis B patients after interferon treatment
Zheng Rui-dan*, Su Ya-yong, Lu Yan-hui, Meng Jia-rong and Zhang Min-feng
Research and Therapy Center for Liver Diseases, Dongnan Hospital affiliated to Xiamen University, Zhang Zhou 363000, Fujian, China
Accepted 09 January, 2019
Abstract
To investigate the intercellular adhesion molecule-1 (ICAM-1) expression in the liver and serum hepatitis B virus (HBV) DNA level in chronic hepatitis B (CHB) patients before and after interferon (IFN) treatment, a total of 51 CHB patients were recruited and treated with IFN- 1b for 6 months. The expressions of ICAM-1 and serum HBV DNA were determined at baseline and after 6-month treatment by using immunohistochemistry and real time fluorescence polymerase chain reaction (PCR). The expression of ICAM-1 in the patients with moderate to severe CHB was markedly higher that that in mild CHB patients (P<0.05) at baseline and after 6-month treatment. Furthermore, IFN treatment could dramatically decrease the ICAM-1 expression among patients with moderate to severe chronic hepatitis B (CHB) (P<0.05). However, the expression of ICAM-1 was relatively stable in patients with mild CHB even after 6-month treatment (P>0.05). In addition, there was a positive correlation between serum level of HBV DNA loads and ICAM-1 expression on hepatocytes in CHB patients, among patients with higher ICAM-1 expression (++, +++, ++++), the serum level of HBV DNA was markedly lowered by IFN- 1b treatment (P<0.05), which was not observed in patients only mildly positive for ICAM -1 (+) . The results indicate that IFN treatment can down-regulate the expression of ICAM-1 in the liver of CHB patients and promote the clearance of HBV, which was more obvious in patients with higher ICAM-1 expression.
Key words: Interferon, hepatitis B, intercellular adhesion molecule-1.