ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-007, February, 2019. © International Scholars Journals
Full Length Research Paper
Isolation and characterization of a novel streptomyces strain Eri11 exhibiting antioxidant activity from the rhizosphere of Rhizoma Curcumae Longae
Kai Zhong1, Xia-Ling Gao 1, Zheng-Jun Xu1*, Li-Hua Li1, Rong-Jun Chen1 Xiao-JianDeng1, Hong Gao2, Kai Jiang1,3 and Isomaro Yamaguchi3
1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, PR, China.
2College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR, China.
3Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Accepted 10 January, 2019
Abstract
In the present study, the phylogenetic analysis of the Streptomyces strain Eri11 isolated from the rhizosphere of Rhizoma Curcumae Longae and the antioxidant activity of the broth cultured with Eri11 were investigated. Analysis of 16S rDNA gene sequences demonstrated that the strains Eri11 was most closely related to representatives of the genera Streptomyces. The total phenols and flavonoids contents in cultured broth were detected to be13.59 ± 0.17 mg gallic acid equivalent/g and 9.93 ± 0.83 mg rutin equivalent/g, respectively. The cultured broth showed the antioxidant activity against the ABTS (2, 2’-Azinobis-3-ethyl benzthiazoline-6-sulfonic acid) free radicals and hydroxyl free radicals with IC50 (The half-inhibitory concentration) of 223.81 ± 24.50 μg/ml and 582.42 ± 83.10 μg/ml respectively. So, it was suggested that the isolated Streptomyces strain Eri11 could be a candidate for the nature resource of the antioxidants.
Key words: Streptomyces, phylogeny, cultured broth, antioxidant activity.