ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Antimicrobial activity of various extracts of Centaurea cankiriense A. Duran and H. Duman
Arzu Cansaran1, Nazime Mercan Do an2*, Mehtap Öztekin3 and Gülümser Acar2
1Department of Biology, Faculty of Education, Amasya University, Amasya, Turkey.
2Department of Biology, Faculty of Science and Arts, Pamukkale University, Denizli, Turkey.
3Ministry of Environment and Forestry, Central Anatolia Forestry Research Institute, Herbarium ANKO, Post Box: 24, Bahcelievler, 06501, Ankara, Turkey.
Accepted 10 March, 2016
Abstract
The antimicrobial activity was determined using the single disc diffusion method. The hexane, methanol and ethyl acetate extracts were assessed for antimicrobial activity against 13 bacteria and a yeast-like fungus, Candida albicans. While flower extracts of Centaurea cankiriense showed significant antibacterial activity against tested strains, the susceptibility of the test microorganisms was less pronounced in the cases of the stem extracts. Hexane extracts from both flower and stem did not show any antibacterial activity against gram-negative bacteria at test concentration, whereas ethyl acetate and methanol extract of C. cankiriense demonstrated the growth of both the gram-positive and the gram-negative bacteria. But, methanol extract inhibited the bacteria with the exception of two gram-negative bacteria namely Escherichia coli and Klebsiella pneumoniae. Minimum inhibition concentration (MIC) was determined on ethyl acetate extracts of flower and stem that showed high activity against the test bacteria. The MIC values for bacterial strains were in the range of 7.8 - 250 mg/ml. The results confirmed that E. coli (MIC = 250 mg/ml) and Morganelle morganii (MIC = 125 mg/ml) was the most resistant organisms to plant extracts. The flower extract of C. cankiriense was found to possess the strongest effect on Bacillus cereus with 7.8 mg/ml concentration.
Key words: Centaurea cankiriense, antimicrobial activity, minimum inhibition concentration, disc diffusion.