International Journal of Medicine and Medical Sciences

ISSN 2167-0404

International Journal of Medicine and Medical Sciences ISSN 2167-0404 Vol. 10 (6), pp. 001-006, June, 2020. © International Scholars Journals

Full Length Research Paper

Study of sensitivity and ability of adenosine deaminase in response to pre-unfolding and especially pathological temperatures via changing the enzyme structure and activity

Mostafa Rezaei-Tavirani1,2*, Saeed Hesami-Takallu3, Seyed Hassan Moghaddamnia1, Shiva Kalantari4, Bijan Ranjbar5, Seyedeh Zahra Moosavi-Nejad6, Sayed-Amir Marashi7, Mohammad Rahmati Roodsari2 and Farhad Malekzad2

1Clinical Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University (M.C.), Tehran, Iran.

2Skin Research Center, Shahid Beheshti University (M.C.), Tehran, Iran.

3Science and Researches Branch, Islamic Azad University, Tehran, Iran.

4Asre-Novin Institute of Research and Industrial Services, Unit 12, No. 38 (Malek Building), Tajrish Sq., Fana-Khosro Ave., Tehran, Iran.

5Department of Biophysics, Faculty of Science, Tarbiat Modarres University, Tehran, Iran.

6Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.

7IMPRS-CBSC, Max Planck Institute for Molecular Genetics, Berlin, Germany.

Accepted 26 April, 2020

Abstract

Adenosine deaminase (ADA) is an important enzyme of the purine metabolic pathway, which catalyzes the conversion of adenosine and deoxyadenosine to their respective inosine derivatives plus ammonia, in a rapid and irreversible reaction. In this work, we studied the structural and kinetic properties of bovine ADA as a function of temperature in the range, 20 - 80°C by circular dichroism (CD) and UV-spectrophotometric techniques, as well as by measuring its activity in this temperature range. The results suggest that thermal unfolding of ADA occurs at temperatures above 60°C, while the enzyme undergoes detectable conformational changes during pre-unfolding heating. These changes affect the kinetics of reaction catalyzed by ADA. The relation between enzyme activity and structural changes is discussed.

Key words: Adenosine deaminase (ADA), UV-Vis spectrophotometry, Conformational changes, Circular dichroism (CD), Kinetic study.

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