ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Full Length Research Paper
Biolistic transformation of Saccharomyces cerevisiae with β-glucosidase gene from Cellulomonas biazotea
S. Parvez*, Z. Mukhtar, F. Rashid, M.I. Rajoka
National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan.
Accepted 27 October 2003
Abstract
A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicating that the gene is expressed in the bombarded yeast.
Key words: Biolistic transformation, β-glucosidase, Cellulomonas biazotea, Saccharomyces cerevisiae.