ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 19 (1), pp. 001-005, January, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Evaluating Growth Regulator Effects on Two Differently Cultured Explants of Carapa guianensis in vitro
Larisse Lobo de Oliveira1, Anaíze Borges Henriques2, Andrea Furtado Macedo1,3*
1Laboratório Integrado de Biologia Vegetal, Departamento de Botânica, Centro de Ciências Biológicas e da Saúde,
Universidade Federal do Estado do Rio de Janeiro, Brazil.
2Laboratório de Fisiologia do Desenvolvimento Vegetal, Departamento de Botânica, Centro de Ciências e da Saúde,
Instituto de Biologia, Universidade Federal do Rio de Janeiro, Brazil.
3Centro de Inovação em Espectrometria de Massas do Laboratório de Bioquímica de Proteínas, Centro de Ciências
Biológicas e da Saúde, Universidade Federal do Estado do Rio de Janeiro, Brazil. *Corresponding author. E-mail: [email protected] Tel: +55 21 2530-2551.
Accepted 14 October, 2022
Abstract
Carapa guianensis Aubl. (Meliaceae), known locally as andiroba, is a multi-use species from Amazonia. Andiroba oil is considered an important natural product in the Brazilian market, and international demand is increasing due to its cosmetic and pharmaceutical potential. C. guianensis trees produce seed irregularly over different harvest periods, leading to inconsistent oil production and difficulties with supply. No management plans or protocols have been developed for in vitro or clonal production of Carapa seedlings and the maintenance of genetic resources. The objective of this study was to assess the effect of growth regulators on explants (young leaves, old leaves and apical buds). Explants consisting of leaf segments 1 cm on a side were cultivated in MS medium with and without growth regulators. Evaluation was based on fresh and dry weight of the explants after 20 days. In the media with 2,4-dichlorophenoxyacetic acid (5, 15, 35 or 45 µM), changes were observed in weight and explant appearance (callus). Bud breakage and development of shoots were achieved using 5 µM of 6-benzylaminopurine. Overall, the results showed that 2,4-dichlorophenoxyacetic acid stimulates callus formation on andiroba foliar explants, while 6-benzylaminopurine was superior to thidiazuron for the initial development of shoots.
Key words: Growth regulators, Carapa guianensis, in vitro, tissue culture, organogenesis.