African Journal of Medicine and Surgery

African Journal of Medicine and Surgery ISSN 3156-8734 Vol. 2 (4), pp. 048-054, October, 2015. © International Scholars Journals

Full Length Research Paper

Effects of carotenoid on the spleen of adult wistar rats following low and high administration

Ovigwe S. Micheal*, Ovedge D. A. and Ejike O. Felix

Department of Anatomy, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.

E-mail: [email protected]

Accepted 22 July, 2015

Abstract

Carotenoids are hypothesized to play a preventive role in a variety of diseases; however, little is known about their biodistribution and metabolism in humans and tissue specific bioactivity. This study is aimed at investigating the effects of carotenoid on the spleen of adult wistar rats following low and high administration. Twenty healthy wistar rats weighing between 150-210 kg were used. They were designated as groups A, B, C and D. Group A served as the control and received 0.35 ml of distilled water; the experimental groups B, C and D received 0.4 ml, 0.5 ml and 0.6 ml of carotenoid respectively for twenty-one days. Twenty four hours after the last administration, the animals were weighed, anaesthetized under the influence of chloroform vapour and dissected. Spleen tissues were removed, weighed and trimmed down to a size of 3 mm × 3 mm and fixed in 10% formaldehyde for histological studies. There was significant weight gain of experimental groups related to the control. The relative spleen weight of the experimental groups was statistically similar with that of the control. Histological finding showed that there was no destruction of the cytoarchitecture of the spleen tissues. These findings suggest that low and high consumption of carotenoid may not put the spleen at risk of adverse histopathological conditions.

Key words: Spleen weight, body weight, carotenoid, antioxidant, hepatoprotective.