ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (6), pp. 261-269, June, 2016. © International Scholars Journals
Full Length Research Paper
A study of phenolic compounds linked to embryogenic structures induction in cotton cell suspension cultures
Kouakou Tanoh Hilaire1, 2 *, Koné Mongomaké2 Kouadio Yatty Justin2, Amani N’guessan Georges2
1Laboratoire de Mycologie et Biotechnologie Végétale, UFR des Sciences Pharmaceutiques, GESVAB, EA 3675, Université de bordeaux 2, 146 rue Léo-Saignat F-33076 Bordeaux cedex, France.
2Laboratoire de Biologie et d’Amélioration des Productions végétales, UFR Sciences de la Nature, Université d’Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire.
*Corresponding author. E-mail: [email protected]. Tel: +225 20304200. Fax: +225 20378118.
Accepted 15 July, 2015
Abstract
Trans-resveratrol is a phytoalexin which was found in some grapes and in many other plants. This phenolic compound has gained much attention, as it was indicated to be associated with fungus and disease resistance. Also, it has been shown that phenols compounds could play an important role in somatic embryogenesis induction of various plants. In cotton, embryogenic structures take place in cell suspension cultures. The need of biochemical indicators for the early identification of cells capable to undergo embryogenic structures formation has always been a major concern of researchers. Trans-resveratrol was found only in embryogenic cell suspensions of Coker 312. This phenolic compound started at the first subculture (2.44 µg/g dw) and the maximum level was reached at the third subculture (7.2 µg/g dw) with an increase of nearly 295%. This study showed a correlation between trans-resveratrol synthesis in cotton cell and embryogenic structures induction. trans-resveratrol may be a phenolic indicator for is induction of cotton somatic embryogenesis.
Key words: Trans-resveratrol, suspension culture, embryogenic structures, cotton, Gossypium hirsutum L.