ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 4 (4), pp. 310-317, April, 2017. © International Scholars Journals
Full Length Research Paper
Extracellular enzymes from brown-rot fungus Laetiporus sulphureus isolated from mangrove forests of coastal Tanzania
Godliving Mtui* and Rose Masalu
Department of Molecular Biology and Biotechnology, University of Dar es Salaam P. O. Box 35179, Dar es Salaam, Tanzania.
*Corresponding author. E-mail: [email protected]. Or [email protected].
Accepted 09 August, 2016
Abstract
A brown-rot fungus, Laetiporus sulphureus (Fr.) Murr., was isolated from Mbweni, Oyster Bay and Mtoni Mangove Forests in Dar es Salaam, Tanzania, and the biochemical properties of its extracellular enzymes were investigated. The crude culture filtrate was concentrated by ultrafiltration. Protein content and lignocellololytic enzyme activities were measured by photometric methods. The crude enzyme extract was purified by gel chromatography and characterized by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The fungal filtrate had maximum manganese peroxidase (MnP) of 2.5 U/mL and lignin peroxidase (LiP) of 1 U/mL, but showed no laccase (Lac) activity. The enzyme extracts were able to oxidize rhemazol brilliant blue-R (RBB-R) dye and phenol, and could remove up to 90% color from raw textile effluent in immobilized culture. The purified peroxidases showed that the MnP from S. sulphureus has a molecular weight of 48 kDA. The study elucidated the extracellular enzymes profile of facultative marine L. sulphureus and provided basic information on their potential for biological wastewater treatment systems.
Key words: Brown-rot fungi, extracellular enzymes, biodegradation, electrophoresis.