ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 9 (7), pp. 001-006, July, 2020. © International Scholars Journals
Full Length Research Paper
Studies on lysine production by Bacillus megaterium
I. A. Ekwealor1* and J. A. N. Obeta2
1Department of Applied Microbiology and Brewing Nnamdi Azikiwe University, Awka Anambra State Nigeria.
2Department of Microbiology University of Nigeria Nsukka, Enugu State, Nigeria.
Accepted 16 May, 2020
Abstract
A Lysine-producing strain recovered from soil was found to produce large amount of the amino acid. The bacterium identified as Bacillus megaterium SP 14 accumulated a lysine yield of 3.56 mg/ml in a broth culture in 96 h. Fermentation experiments show that 8.0% (w/v) glucose and 4.0% (w/v) ammonium chloride used as sources of carbon and nitrogen, respectively, in a medium/fermenter volume ratio of 25.0%, influenced accumulation of the amino acid. Amino acids other than the aspartate family at 0.01% (w/v) stimulated growth and improved lysine yield. Addition of 0.01 unit/ml penicillin to the fermentation medium, immediately after inoculation, stimulated growth and appreciably enhanced lysine accumulation.
Key words: Lysine, amino acid, Bacillus megaterium, fermentation, penicillin.