ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-007, March, 2019. © International Scholars Journals
Full Length Research Paper
Biochemical and molecular characterization of hemolytic Bacillus licheniformis strains isolated from shrimp and clam aquacultures
Nermeen A. ElSersy1* and Eman A. H. Mohamed2
1National Institute of Oceanography and Fisheries, Microbiology laboratory, Environmental Division, Alexandria, Egypt.
2Botany Department, Faculty of Science, Damanhour University, Egypt.
Accepted 15 January, 2019
Abstract
A polyphasic approach was used to discriminate among the significantly hemolytic and closely related Bacillus licheniformis strains, NE-1, NE-2 and NE-3. Utilization of the API 20E kit revealed that B. licheniformis NE-1 is unique in its ability to utilize sorbitol as a sole carbon source, whereas B. licheniformis NE-2 is an exceptional in L-arabinose utilization. The phylogenetic analysis using the 16S rDNA 5´end revealed a strong relatedness to B. licheniformis strains found in the GenBank with a similarity percentage ranging from 97 to 99. Moreover, the partial sequences was successfully differentiated among the novel strains. The plasmid pattern is almost the same for the strains and plasmid curing using acridine orange showed no effect on the hemolytic activity of them.
Key words: Bacillus licheniformis, aquacultures, hemolytic activity, plasmids, 16S rDNA.