ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-006, December, 2018. © International Scholars Journals
Full Length Research Paper
Purification and characterization of chitinase from Micrococcus sp.AG84 isolated from marine environment
N. Annamalai*, S. Giji, M. Arumugam and T. Balasubramanian
Centre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai 608502, India.
Accepted 10 October, 2018
Abstract
Chitinase producing bacterial strain Micrococcus sp. AG84 isolated from marine sediments grew maximally in shake flask and produced chitinase at 35°C, pH 8.0. Chitinase activity was found to be maximum at 45°C, pH 8.0, and the enzyme was 100% stable even at 60°C and pH 11.0. Added with Fe2+, Ca2+ and Ni2+ chitinase activity increased but it was inhibited by EDTA. The molecular weight of purified chitinase is 33 kDa.
Key words: Micrococcus sp. AG84, chitinase, thermostable, alkaline, marine.