ISSN 2997-1036
International Journal of Hematology | Vol. 10, No. 11, November 2019 | pp. 81–88
DOI: 10.46882/2019/IJH/000119
Original Article
Title: Immunophenotypic profile and clinical stage stratification of mature B-cell lymphoproliferative disorders utilizing CD200 and CD27 expression variations
Names of Authors: W. A. Adebayo¹, X. Y. Emeka², Z. Z. Salami³
Authors’ Affiliations: ¹Department of Haematology, University of Ibadan, Ibadan, Nigeria; ²Department of Haematology, University of Calabar Teaching Hospital, Calabar, Nigeria; ³Department of Pathology, Lagos University Teaching Hospital, Lagos, Nigeria
Abstract: Multiparameter flow cytometry immunophenotyping panels play an important role in separating overlapping mature B-cell malignancies. This prospective study evaluated the diagnostic performance of combining CD200 and CD27 markers to differentiate chronic lymphocytic leukemia from mantle cell lymphoma and marginal zone lymphoma in 55 adult patients presenting with persistent absolute lymphocytosis. Lineage markers and monoclonal light chain restriction were established using flow cytometry. Chronic lymphocytic leukemia was confirmed in 38 cases, while 17 were diagnosed with non-CLL mature B-cell variants. Strong, uniform surface co-expression of CD200 and CD27 was detected in 94.7% (36 of 38) of the chronic lymphocytic leukemia cases. In contrast, mantle cell lymphoma cohorts demonstrated a complete absence of both markers alongside bright CD20 expression (P < 0.001). Marginal zone lymphoma variants exhibited positive CD27 paired with negative or dim CD200 parameters. High expression density for both markers correlated with early clinical presentation (Binet Stage A). Incorporating CD200 and CD27 into standard screening protocols provides excellent diagnostic specificity, reducing borderline scores and helping classify mature B-cell expansions.
Keywords: Chronic lymphocytic leukemia, CD200, CD27, flow cytometry, lymphoproliferative disorders, immunophenotyping
Manuscript Timeline: Received: August 20, 2019; Revised: September 25, 2019; Accepted: October 12, 2019; Published: November 15, 2019