ISSN 2169-3048
International Journal of Biochemistry and Biotechnogy ISSN:2169-3048 Vol. 1 (6), pp. 171-174, August, 2012. © International Scholars Journals
Full Length Research Paper
Embryogenetic studies of intra-specific clarias anguillaris hybrid: Implication on genetic manipulation
*Onyia, L.U.1 Ladu, B.M.B.2 and Olufeagba, S.O3.
1Department of Fisheries, Modibbo Adama University of Technology, P.M.B.2076, Yola Nigeria.
2Department of Biological Sciences, Modibbo Adama University of Technology, P.M.B.2076, Yola Nigeria.
3Aquaculture and Biotechnology centre, National Institute for Freshwater Fisheries Research P.M.B.6006, New Bussa, Niger State.
*Correspondence author E-mail: [email protected]
Received 12 July, 2012; Accepted 24 August, 2012
Abstract
The need to carry out a thorough study on the embryogenesis of intra-specific Clarias anguillaris to determine the best time and the implication in chromosome engineering research cannot be over-emphasized. In this study, it was observed that the first cleavage into 2-cell stage was 34minutes after fertilization, which gave the right timing for the production of tetraploids. The 4-cell, 8-cell and 16-cell stages occurred at 47 minutes, 52 minutes and 53 minutes respectively. Fifty-four percent (54%) of the embryo attained morula stage at 94 minutes. Other embryonic stages observed include blastula, gastrulation, somite formation and hatching. Hatching started at 18hours after fertilization and lasted for a period of about 6hours. The hatchlings increased in length at 0.2mm/day in the first 5 days after hatching.
Key words: Embryonic, development, intra-specific, Clarias anguillaris.