ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 2 (3), pp. 130-135, March, 2015. © International Scholars Journals
Full Length Research Paper
A study of flower chimera and the special cytological behavior that happened in the hybrids with flower chimera
Jianmin Liu1,3, Hao Wang2, Longjiang Yu1,3, Dianrong Li2* and Maoteng Li1,3*
1Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
2Hybrid Rapeseed Research Center of Shanxi Province, Dali 715105, China.
3College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
*Corresponding author. E-mail: [email protected] or [email protected].
Accepted 21 November, 2014
Abstract
Hybridization between white flowered Brassica carinata and yellow flowered B. rapa were made, and the flower chimeras were observed in a few hybrids. The simple single sequence molecular markers verified the hybridity of those hybrids. Chimeras were justified and totally classified based on the morphological characteristics of the flower petals that appeared in the hybrids of B. carinata and B. rapa. Two kinds of flower chimeras were observed: one type was different flower petals were with different colour in one branch; another type was that yellow petals were with white variegations, but the variegation size and shape were different in different petals. The meiosis and mitosis analysis showed that the partial or complete separation of parental genomes inferred to occur in pollen mother cells, shoot and early-developed petals in the flower chimeral hybrids, which hinted that the occurrence of complete or partial segregation of parental genomes in the somatic cells might be the reason for the production of flower chimera in the hybrids of B. carinata and B. rapa.
Key words: Brassica carinata, B. rapa, SSR, flower chimera.