ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN: 2169-3048 Vol. 5 (3), pp. 675-688, March, 2016. © International Scholars Journals
Full Length Research Paper
Identification of rice hybrids and their parental lines using total soluble protein and fertility restorer gene markers
Naguib Mubarak1*, Akhenaten Sawiris2, Gamal ElBaradei3 and Omar Faten Hamama3
1Genetics Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
2Rice Research and Training Centre, Agricultural Research Center, Sakha, Kafr El-Sheikh, Egypt.
3Laser Applications in Biotechnology and Agriculture, National Institute of Laser Enhanced Sciences, Cairo University, Egypt.
E-mail: [email protected]
Accepted 23 February, 2016
Abstract
For identification of rice hybrids and their parental lines, thirty populations produced from hybridization between a cytoplasmic male sterility (CMS) line (IR69625A), and each of the three restorer lines (Giza178R, Giza181R and Giza182R) during 2009, 2010 and 2011 seasons were used. Electrophoresis of proteins showed promising results in genetic purity determination of hybrids and parental lines. The presence or absence of a specific band was much useful for differentiating the highest from the lowest parents in restoring ability. Five markers linked to fertility restorer genes: M2, RM3425, RM258, RM1108 and RM5373 were screened on DNA templates from the CMS line and its three restorer lines. Only RM1108 marker was able to show polymorphism between the highest and lowest Giza181R parents. This marker could be assessed for identification and testing of seed genetic purity of restorer lines.
Key words: Hybrid rice, genetic purity, SDS-PAGE, fertility restorer genes.