ISSN 2329-9800
International Journal of Microbiology Research and Reviews ISSN 2329-9800 Vol. 5 (3), pp. 175-183, March, 2016. © International Scholars Journals
Full Length Research Paper
A morphological, physiological and molecular investigation of fischerella sp. MCCS023 (cyanobacteria) from paddy-fields of Iran
1Siahbalaei R, 2Soltani N, 3Shokravi SH, and 4Afsharzadeh S
1Dep. Biology, Faculty Science, Isfahan University, Isfahan, 2Dep. Petroleum Microbiology, ACECR, Research Institute of Applied Science, Tehran, Iran, 3Dep. Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran, 4Dep. Biology, Faculty Science, Isfahan University, Isfahan.
*Corresponding author E-mail: [email protected]
Accepted 02 February, 2015
Abstract
Fischerella ambigua is an important member of the cyanobacterial community in the rice field. In this study morphological, taxonomical variation in blue-green algae Fischerella ambigua was determined. In later stage they are treated with different nitrogen sources (N-free, 1 & 2 mM NH4+ and 5 and 10 mM NO3−). Results indicated that maximum growth rate belonged to NO3− treatment. Dry weights of Fischerella sp. MCCS023 in N-free medium and ammonium were in decreasing order of preference; and NH4 + 2 mM was drastically toxic for this strain and at last molecularly investigated. For Genetic variation, among rice-field, Fischerella isolates were studied using DNA extracted from single filament-derived cultures. Laboratory studies have shown that it is possible to use PCR to amplify individual Cyanobacteria filaments. As a result of these studies, Shiraz University was isolated for direct PCR and sequence analysis. Our results show that two distinct genotypes of Fischerella are present in the population at all stations and are not present in approximately equal numbers, they are not distributed uniformly.
Keywords: Ammonium, cyanobacteria, fischerella ambigua, Iran, nitrate, molecular investigation, paddy-fields, taxonomy.