ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN 7295-2849 Vol. 2 (5), pp. 001-005, May, 2012. © International Scholars Journals
Full Length Research Paper
Assessment of a fruit extract (Sechium edule) on the labeling of blood elements with technetium-99m
Gláucio Diré Feliciano1*, Maria Luísa Gomes1, Elaine Alves Correia Lima1, Roberto Levi Jales3, Mauro Castro Faria1 and Mário Bernardo Filho2
1Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de
Biofísicae Biometria. Av. 28 de Setembro, 87, Rio de Janeiro, RJ 20551-030, Brazil.
2Instituto Nacional do Câncer, Centro de Pesquisa Básica, Praça Cruz Vermelha, 23, CEP 20230-130, Rio de Janeiro, RJ, Brazil.
3Universidade Federal do Rio Grande do Norte, Departamento de Farmácia, Natal, Rio Grande do Norte, Brazil.
Accepted 06 January, 2012
Abstract
Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. Chayotte (Sechium edule) is a vegetable used in the folk medicine. Red blood cells (RBC) labeled with technetium-99m (99mTc) have several clinical applications. The aim of this work was to evaluate the influence of an extract of chayotte on the labeling of blood elements with 99mTc using stannous chloride (SnCl2) in the concentrations like to 1.2, 0.006, 0.0005 and 0.0006 µg/ml. The extract of chayote was incubated in various concentrations for 1hour with blood which was withdrawn from Wistar rats. After that SnCl2 was added and the incubation continued for more 1 h. Elapsed this time 99mTc as sodium pertechnetate (NaTcO4) was toted. The blood was centrifuged and plasma (P) and RBC were isolated, also precipitated with trichloroacetic acid (TCA, 5%) and soluble (S) and insoluble (I) fractions (F) of plasma and cells (C) were determined. The radioactivity (ATI%) was rated in RBC, IF-P and IF-C. The results have showed that extract was able to reduce the radiolabeling using SnCl2 (0.006, 0.0005 and 0.0006µg/ml). We can speculate that this effect may be on account of the products with oxidant proprieties.
Keywords: chayote, red blood cells, plasma proteins, technetium-99m, radiopharmacy.